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Sex was not considered as a biological variable in the primary study design because (i) cyclical hormonal variation in female mice is a known confounder of LPS-induced inflammatory cytokine responses, and (ii) the C57BL/6J LPS doseresponse and time course on which our paradigm was based has been characterized primarily in males in prior literature

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Underwood CR, Parthier C, Reedtz-Runge S (2010) Structural basis for ligand recognition of incretin receptors